Poly(ADP-ribose) synthase is the major endogenous nonhistone acceptor for poly(ADP-ribose) in alkylated rat hepatoma cells

Abstract

The endogenous poly(ADP‐ribosyl)‐nonhistone protein conjugates were isolated from dimethyl‐sulfate‐treated rat hepatoma AH 7974 cells using aminophenylboronic‐acid‐agarose chromatography. Seven major components could be discerned on dodecyl sulfate gels (molecular mass 43, 60, 66, 86, 100, 110 and 170 kDa) while control cells indicated only slight staining at above 200 kDa. The most abundant conjugate formed in response to alkylation damage was further purified using preparative gel electrophoresis and identified on the basis of its intrinsic enzymic activity as automodified poly(APD‐ribose) synthase. In addition, topoisomerase I activity was found associated with a 60‐kDa peptide. ADP‐ribosylated endonucleases and actin were not detectable. The purified conjugate fraction contained maximally 8.8 nmol/mg ADP‐ribose and 7.9 nmol/mg oligo(ADP‐ribose) with a mean chain length of 2.3 residues. The modifying (ADP‐ribosyl)_n groups were attached to its acceptors by a hydroxylamine‐insensitive bond and had practically no effect on the DNA affinity of either poly(ADP‐ribose) synthase or topoisomerase I.