Chromatin condensation in terminally differentiating mouse erythroblasts does not involve special architectural proteins but depends on histone deacetylation
- 27 January 2009
- journal article
- research article
- Published by Springer Nature in Chromosome Research
- Vol. 17 (1), 47-64
- https://doi.org/10.1007/s10577-008-9005-y
Abstract
Terminal erythroid differentiation in vertebrates is characterized by progressive heterochromatin formation and chromatin condensation and, in mammals, culminates in nuclear extrusion. To date, although mechanisms regulating avian erythroid chromatin condensation have been identified, little is known regarding this process during mammalian erythropoiesis. To elucidate the molecular basis for mammalian erythroblast chromatin condensation, we used Friend virus-infected murine spleen erythroblasts that undergo terminal differentiation in vitro. Chromatin isolated from early and late-stage erythroblasts had similar levels of linker and core histones, only a slight difference in nucleosome repeats, and no significant accumulation of known developmentally regulated architectural chromatin proteins. However, histone H3(K9) dimethylation markedly increased while histone H4(K12) acetylation dramatically decreased and became segregated from the histone methylation as chromatin condensed. One histone deacetylase, HDAC5, was significantly upregulated during the terminal stages of Friend virus-infected erythroblast differentiation. Treatment with histone deacetylase inhibitor, trichostatin A, blocked both chromatin condensation and nuclear extrusion. Based on our data, we propose a model for a unique mechanism in which extensive histone deacetylation at pericentromeric heterochromatin mediates heterochromatin condensation in vertebrate erythroblasts that would otherwise be mediated by developmentally-regulated architectural proteins in nucleated blood cells.Keywords
This publication has 75 references indexed in Scilit:
- Global modulation of chromatin dynamics mediated by dephosphorylation of linker histone H1 is necessary for erythroid differentiationProceedings of the National Academy of Sciences, 2006
- The X and Y Chromosomes Assemble into H2A.Z, Containing Facultative Heterochromatin, following MeiosisMolecular and Cellular Biology, 2006
- Cathepsin L Stabilizes the Histone Modification Landscape on the Y Chromosome and Pericentromeric HeterochromatinMolecular and Cellular Biology, 2006
- The end adjusts the means: Heterochromatin remodelling during terminal cell differentiationChromosome Research, 2006
- The epigenetic progenitor origin of human cancerNature Reviews Genetics, 2006
- The reorganisation of constitutive heterochromatin in differentiating muscle requires HDAC activityExperimental Cell Research, 2005
- Translating the Histone CodeScience, 2001
- Transitions in histone acetylation reveal boundaries of three separately regulated neighboring lociThe EMBO Journal, 2001
- A novel nonhistone protein (MENT) promotes nuclear collapse at the terminal stage of avian erythropoiesisExperimental Cell Research, 1992
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970