Minor Collagens in Arthrotic Human Cartilage: Change in Content of 1α, 2α, 3α and M-collagen with Age and in Osteoarthrosis
- 1 January 1983
- journal article
- research article
- Published by Medical Journals Sweden AB in Acta Orthopaedica
- Vol. 54 (4), 613-619
- https://doi.org/10.3109/17453678308992898
Abstract
The content and distribution of 1α, 2α, 3α and M-collagens in human articular cartilage were studied. As controls, normal femoral heads and costal cartilage of autopsy material from newborn to 91-year-old persons were used. The osteoarthrotic cartilage was obtained from patients undergoing total hip replacement aged 45-80. The pepsin-digested cartilage collagen was fractionated by differential salt fractionation. The collagen content of the fractions was determined, and the fractions were separated by polyacrylamide slab gel electrophoresis. In the extracted collagen, the type II collagen varied from 82 to 97 per cent with increasing age. The 1α, 2α and 3α chains decreased. M-collagen, especially of the high molecular weight components, disappeared with age. In ostearthrosis three types of change - degeneration, new fibrocartilage formation on the surface of osteophyte and reparative cartilage - were separately studied. In all types of osteoarthrosis, an increase of minor collagens was found. In newly formed fibrocartilage, the reappearance of M-collagen was conspicuous. It is proposed that the three types of osteoarthrotic cartilage may be characterized on the basis of content and distribution of minor collagens.Keywords
This publication has 10 references indexed in Scilit:
- Human cartilage collagens. Comparison of cartilage collagens with human type V collagen.Journal of Biological Chemistry, 1982
- Analysis of the Type of Collagen Present in Osteoarthritie Human CartilagePublished by Wolters Kluwer Health ,1982
- Isolation and Characterization of New Collagens from Chick CartilageEuropean Journal of Biochemistry, 1982
- Characterization of a highly soluble collagenous molecule isolated from chicken hyaline cartilageBiochemistry, 1982
- Minor collagens of chicken hyaline cartilageBiochemistry, 1981
- Isolation and characterisation of an unusual collagen from hyaline cartilage and intervertebral discFEBS Letters, 1981
- Collagen molecules comprised of α1(V)-chains (B-chains): an apparent localization in the exocytoskeletonCollagen and Related Research, 1980
- Identification of a new disulphide bonded collagen from cartilageFEBS Letters, 1980
- Collagen heterogeneity in human cartilage: Identification of several new collagen chainsBiochemical and Biophysical Research Communications, 1979
- Cartilage collagen in osteoarthrosisClinica Chimica Acta; International Journal of Clinical Chemistry, 1976