The sequence of human serum albumin cDNA and its expression in E. coli
- 1 January 1981
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (22), 6103-6114
- https://doi.org/10.1093/nar/9.22.6103
Abstract
A recombinant plasmid has been constructed which contains the mature protein coding region of the human serum albumin (HSA) gene. Bacteria containing this plasmid synthesize HSA protein under control of the E. coli trp promoter-operator. The DNA sequence and predicted protein sequence of HSA were determined from the cDNA plasmid and are compared to existing data obtained from direct protein sequencing. The DNA sequence predicts a mature protein of 585 amino acids preceded by a 24 amino acid "prepro" peptide.Keywords
This publication has 35 references indexed in Scilit:
- The evolution of alpha-fetoprotein and albumin. II. The structures of the alpha-fetoprotein and albumin genes in the mouse.Journal of Biological Chemistry, 1981
- The evolution of alpha-fetoprotein and albumin. I. A comparison of the primary amino acid sequences of mammalian alpha-fetoprotein and albumin.Journal of Biological Chemistry, 1981
- A system for shotgun DNA sequencingNucleic Acids Research, 1981
- Human leukocyte interferon produced by E. coli is biologically activeNature, 1980
- Synthesis of human fibroblast interferon by E. coliNucleic Acids Research, 1980
- Synthesis of oligonucleotides on cellulose by a phosphotriester methodNucleic Acids Research, 1980
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesMethods in Enzymology, 1980
- A rapid alkaline extraction procedure for screening recombinant plasmid DNANucleic Acids Research, 1979
- Direct expression in Escherichia coli of a DNA sequence coding for human growth hormoneNature, 1979
- Biosynthesis of Bovine Plasma Proteins in a Cell‐Free SystemEuropean Journal of Biochemistry, 1979