Active Enzyme Preparations from Bacteria

Abstract

A semi-mechanical adaptation of the glass-grinding method for the prepn. of bacterial cell-free extracts is described. The method involves the passage of a paste of bacteria and powdered glass between concentric cones of heavy glass. The inner cone is revolved by a motor while the outer cone is held firmly in place. After grinding, the resulting paste is extracted with buffer soln. or water and the extract freed of glass, intact cells, and cell fragments by centrifugation.