Characterization of a Cytosolic Aconitase in Higher Plant Cells
Open Access
- 1 August 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 84 (4), 1402-1407
- https://doi.org/10.1104/pp.84.4.1402
Abstract
Protoplasts obtained from sycamore (Acer pseudoplatanus) cell suspensions were found to be highly intact. If the protoplasts were taken up and expelled through a fine nylon mesh, all the protoplasts were ruptured leaving the fragile amyloplasts largely intact. Aconitase hydratase (citrate [isocitrate] hydro-lyase, EC 4.2.1.3) activity of sycamore cells was associated with two protein fractions, one present in the cytosol while the second is of mitochondrial origin. Chromatography on DEAE-trisacryl did not separate the aconitase hydratase isoenzymes. EPR studies established that both isoenzymes exhibited an EPR signal at g = 2.03 once oxidized.This publication has 20 references indexed in Scilit:
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