High-order fluorescence fluctuation analysis of model protein clusters.
- 1 August 1989
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (16), 6148-6152
- https://doi.org/10.1073/pnas.86.16.6148
Abstract
The technique of high-order fluorescence fluctuation autocorrelation for detecting and characterizing protein oligomers was applied to solutions containing two fluorescent proteins in which the more fluorescent proteins were analogues for clusters of the less fluorescent ones. The results show that the model protein clusters can be detected for average numbers of observed subunits (free monomers plus monomers in oligomers) equal to 10-100 and for relative fluorescent yields that correspond to oligomers as small as trimers. High-order fluorescent fluctuation analysis may therefore be applicable to cell surface receptor clusters in natural or model membranes.Keywords
This publication has 15 references indexed in Scilit:
- Signal Transduction by the Platelet-Derived Growth Factor ReceptorScience, 1989
- Polarized fluorescence photobleaching recovery for measuring rotational diffusion in solutions and membranesBiophysical Journal, 1988
- Molecular aggregation characterized by high order autocorrelation in fluorescence correlation spectroscopyBiophysical Journal, 1987
- Epidermal growth factor induces rapid, reversible aggregation of the purified epidermal growth factor receptorBiochemistry, 1987
- Molecular counting of low-density lipoprotein particles as individuals and small clusters on cell surfacesBiophysical Journal, 1986
- Scanning fluorescence correlation spectroscopy. II. Application to virus glycoprotein aggregationBiophysical Journal, 1986
- T-cell-mediated association of peptide antigen and major histocompatibility complex protein detected by energy transfer in an evanescent wave-fieldNature, 1986
- Role for mouse macrophage IgG Fc receptor as ligand-dependent ion channelNature, 1983
- Structural Basis of Antibody FunctionAnnual Review of Immunology, 1983
- Fluorescence correlation spectroscopy. II. An experimental realizationBiopolymers, 1974