Calcium-Lipid Complexes in Human Platelets
Open Access
- 1 June 1958
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 13 (6), 589-598
- https://doi.org/10.1182/blood.v13.6.589.589
Abstract
Carefully washed human platelets contain calcium in an unusual state of chemical combination. Platelet calcium is non-ionic and is not exchangeable with ionic Ca45. On extraction of platelets with lipid solvents, the calcium separates with the phospholipids. The calcium-lipid complex thus obtained does not undergo any exchange with radioactive calcium. Study of other properties of platelets reveals that the clot-promoting activity of platelets (activated by antihemophilic factor and plasma thromboplastin component) also separates into the lipid fraction. Preliminary observations are reported on the nature of platelet lipids as revealed by paper chromatography and paper electrophoresis in solvents of low dielectric constant. Small amounts of proteolipid have been isolated from the lipid extracts. The unique calciumlipid complex is thought to originate in the cell membrane of the platelet.Keywords
This publication has 8 references indexed in Scilit:
- THE INCORPORATION IN VIVO OF P32-LABELED ORTHOPHOSPHATE INTO INDIVIDUAL PHOSPHATIDES OF RAT TISSUESJournal of Biological Chemistry, 1957
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957
- Blood Coagulation: With Particular Reference to the Early StagesPhysiological Reviews, 1956
- THE QUANTITATIVE HISTOCHEMISTRY OF BRAIN .1. CHEMICAL METHODS1954
- PROTEOLIPIDES, A NEW TYPE OF TISSUE LIPOPROTEINS - THEIR ISOLATION FROM BRAIN1951
- BRAIN DIPHOSPHOINOSITIDE, A NEW PHOSPHATIDE HAVING INOSITOL METADIPHOSPHATE AS A CONSTITUENTJournal of Biological Chemistry, 1949
- SIGNIFICANCE OF THE CELL MEMBRANE IN EMBRYONIC PROCESSESAnnals of the New York Academy of Sciences, 1948
- Capillary endothelial cement in relation to permeabilityJournal of Cellular and Comparative Physiology, 1940