Furin is important but not essential for the proteolytic maturation of gp160 of HIV‐1
- 22 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 365 (1), 95-97
- https://doi.org/10.1016/0014-5793(95)00447-h
Abstract
The envelope glycoproteins of HIV are required for viral infectivity. Proteolysis of the precursor envelope glycoprotein gp160 results in the formation of gp120 and gp41. Cleavage occurs after the sequence Arg-Glu-Lys-Arg. This sequence is expected to be a substrate for the cellular protease furin. We examined whether furin is responsible for cleavage of gp160 by using a furin-deficient CHO cell line and the same cell line transfected with furin cDNA. Data obtained from viral transmission assays suggested that furin increased viral infectivity but was not essential for the maturation of gp160, implying that other proprotein processing enzymes also recognize this putative furin cleavage site.Keywords
This publication has 34 references indexed in Scilit:
- Evidence for involvement of furin in cleavage and activation of diphtheria toxin.Journal of Biological Chemistry, 1993
- Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gpl60Nature, 1992
- Characterization of an HIV-1 point mutant blocked in envelope glycoprotein cleavageVirology, 1990
- Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virusCell, 1988
- Induction of CD4-dependent cell fusion by the HTLV-III/LAV envelope glycoproteinNature, 1986
- Nucleotide sequence of a full-length cDNA for mousecytosketetal β-actin raRNANucleic Acids Research, 1986
- The trans-activator gene of HTLV-III is essential for virus replicationNature, 1986
- Characterization of gp41 as the Transmembrane Protein Coded by the HTLV-III/LAV Envelope GeneScience, 1985
- T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAVNature, 1984
- The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirusNature, 1984