Induction of cadmium-thionein in isolated rat liver cells
- 15 February 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 170 (2), 219-225
- https://doi.org/10.1042/bj1700219
Abstract
The uptake of Cd by isolated liver cells was linearly related to the Cd concentration to which the cells were exposed in the medium. Cd-treated cells synthesized proteins de novo with the characteristics of Cd-thionein induced in the liver of Cd-treated animals. Thionein from liver cells incorporated Cd and [35S]cysteine, had a Ve/Vo [elution volume/void volume] (Sephadex G-50) of 1.8-1.9, and was separated into 2 subfractions by DEAE-cellulose ion-exchange chromatography. Cycloheximide and actinomycin D when added after a Cd exposure prevented the synthesis of thionein. Addition of actinomycin D after synthesis had started only decreased the total amount of thionein synthesized. The concentration of Cd to which the cells were exposed affected the amount of Cd-thionein synthesized in 6 h. The maximum response occurred when cells were exposed to 0.5 .mu.g of Cd/ml; at higher metal concentrations the total amount of Cd-thionein synthesized declined. This system can be used to study the mode of metal toxicity and the mechanism of Cd-thionein synthesis.This publication has 36 references indexed in Scilit:
- Metallothionein synthesis as an indication of the parenchymal origin of cells cultured from liverChemico-Biological Interactions, 1976
- The biosynthesis of metallothionein in rat liver and kidney after administration of cadmiumChemico-Biological Interactions, 1976
- DNA synthesis and mitotic activity in short-term cultures of hepatocytes isolated from regenerating rat liverExperimental Cell Research, 1976
- Effect of cadmium on RNA-polymerase and protein synthesis in rat liverFEBS Letters, 1976
- Nuclear 115cadmium: Uptake and disappearance correlated with cadmium-binding protein synthesisBiochemical and Biophysical Research Communications, 1976
- Biochemical changes in rat kidney on exposure to elemental mercury vapor: Effect on biosynthesis of metallothioneinChemico-Biological Interactions, 1976
- Mammalian zinc homeostasis: Requirement for RNA and metallothionein synthesisBiochemical and Biophysical Research Communications, 1975
- Influence of parenteral zinc and actinomycin D on tissue zinc uptake and the synthesis of a zinc-binding proteinBioinorganic Chemistry, 1975
- Isolation and purification of cadmium binding proteins from rat liverBiochemical and Biophysical Research Communications, 1974
- Cadmium‐induced synthesis of hepatic metallothionein in chicken and ratsFEBS Letters, 1973