Gene transfer in primary cultures of human hepatocytes
- 1 May 1992
- journal article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology – Animal
- Vol. 28 (5), 373-375
- https://doi.org/10.1007/bf02877062
Abstract
Using liposomes as the mediator of DNA transfer, we were successful in the transfection of human hepatocytes isolated from surgical samples with an E. coli β-galactosidase gene (β-gal). A comparison of transfection efficiency showed that of the four promoters used, cytomegalovirus (CMV) promoter yielded higher transfection efficiencies than Rous sarcoma virus (RSV), Simian virus-40 (SV-40) and human alpha-l antitrypsin (AAT) promoters. These studies represent the first report on the successful transfection of primary cultures of human hepatocytes.Keywords
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