Studies of Coxsackie Virus: Adaptation of a Strain to Chick Embryos

Abstract

Following the use of an alternating mouse-to-egg passage technique, a Coxsackie virus similar to Dalldorf''s Type 2 was propagated in 10 consecutive yolksac passages. Despite the fact that inoculums were injd. into the yolksac cavity, nearly all demonstrable virus localized in embryo tissue after 10 passages. Chick embryo tissues when diluted 10-7 (original mouse by inoculum ID50 = 7.2) produced paralysis and death in mice. After 5, 7, 8 and 9 passages the egg-propagated virus was neutralized in a specific manner by antiserum prepared against Dalldorf''s Type 2 and homologous mouse passage virus.