ANTIGENIC RELATIONSHIPS OF BENCE JONES PROTEINS, MYELOMA GLOBULINS, AND NORMAL HUMAN γ-GLOBULIN
Open Access
- 1 January 1963
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 117 (1), 81-104
- https://doi.org/10.1084/jem.117.1.81
Abstract
By means of immunodiffusion and immunoelectrophoresis study has been made of antigenic relationships of Bence Jones proteins, and the three classes of normal and pathological immunoglobulins, 7S gamma, beta(2A), and beta(2M). All thirty-nine Bence Jones proteins studied could be classified into either one of two distinct antigenic types, A or B. Both types are related to the immunoelectrophoretically slow (S) fragment of a papain digest of normal gamma-globulin; B is related more closely than A, but neither has antigenic determinants in common with the fast (F) fragment. The 7S gamma myeloma globulins were either immunological type I or II. The papain digests of these proteins produced the S and F precipitin lines in immunoelectrophoresis but multiple bands in starch gel electrophoresis, especially in the F region. The S fraction of type I myeloma globulins is antigenically similar to Bence Jones protein of type B, and the S component of type II myeloma globulins has antigenic determinants in common with type A Bence Jones protein. Correspondingly, myeloma patients with type I globulins and proteinuria usually excrete type B Bence Jones proteins, whereas patients with type II excrete type A proteins. The F fragment is the part common to normal 7S gamma-globulin and types I and II myeloma globulins but is absent in beta(2A) and beta(2M) pathological globulins and in both types of Bence Jones proteins. Papain digests of beta(2A) myeloma globulins produced a single precipitin line in immunoelectrophoresis. beta(2A) myeloma globulins appeared to have two antigenic units, one in common with type B Bence Jones protein and normal gamma-globulin, and another specific to beta(2A). The beta(2A) myeloma patients excreted type B Bence Jones protein. The papain digest of a macroglobulin produced two precipitin lines, the faster of which had antigenic determinants in common with type B Bence Jones protein, the slower seemed specific for the macroglobulin. Five serum micromolecular globulins proved to be either type A or B Bence Jones proteins. From the above results, an antigenic map was constructed showing which determinants are shared and which are specific for normal 7S gamma-globulin, types I and II myeloma globulins, beta(2A) myeloma globulins, a macroglobulin, and types A and B Bence Jones proteins.Keywords
This publication has 22 references indexed in Scilit:
- ENZYMATICALLY PRODUCED SUBUNITS OF PROTEINS FORMED BY PLASMA CELLS IN MICEThe Journal of Experimental Medicine, 1962
- ENZYMATICALLY PRODUCED SUBUNITS OF PROTEINS FORMED BY PLASMA CELLS IN MICEThe Journal of Experimental Medicine, 1962
- ANTIGENIC RELATIONSHIPS BETWEEN IMMUNE GLOBULINS AND CERTAIN RELATED PARAPROTEINS IN MANThe Journal of Experimental Medicine, 1961
- CLEAVAGE OF HUMAN GAMMA-GLOBULIN BY PAPAIN1961
- DETECTION IN NORMAL URINE OF PROTEIN RESEMBLING BENCE JONES PROTEINJCI Insight, 1960
- Human Gamma Globulin Fractionation on Anion Exchange Cellulose ColumnsJournal of Biological Chemistry, 1959
- An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individualsBiochemical Journal, 1959
- ABNORMAL HUMAN SERUM GLOBULINS .2. PHYSICAL CONSTANTS AND AMINO END GROUPS1958
- Starch Gel Electrophoresis in a Discontinuous System of BuffersNature, 1957
- Aberrations of Protein Metabolism in Multiple Myeloma: Interrelationships of Abnormal Serum Globulins and Bence-Jones ProteinsPhysiological Reviews, 1957