Comparative biochemical properties of normal and activated human ras p21 protein
- 1 August 1984
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 310 (5979), 644-649
- https://doi.org/10.1038/310644a0
Abstract
Human Ha-ras1 c[complementary]DNA encoding normal and activated p21 polypeptides were efficiently expressed in Escherichia coli and the biochemical activities associated with each polypeptide compared. In addition to the guanine nucleotide binding activity, normal p21 displays a GTPase activity which is selectively impaired by a mutation which activates its oncogenic potential.This publication has 52 references indexed in Scilit:
- High-Level Expression in Escherichia coli of Enzymatically Active Harvey Murine Sarcoma Virus p21
ras
ProteinScience, 1983
- Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutationsNature, 1983
- Nucleotide Sequence Analysis of the T24 Human Bladder Carcinoma OncogeneScience, 1983
- Altered gene products are associated with activation of cellular rasK genes in human lung and colon carcinomasCell, 1983
- The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipidCell, 1982
- Bacterial expression of an enzymatically active protein encoded by RSV src geneNature, 1982
- Human leukocyte interferon produced by E. coli is biologically activeNature, 1980
- Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistryCell, 1980
- Transforming activity of DNA of chemically transformed and normal cellsNature, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979