Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor
- 23 March 1984
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 223 (4642), 1296-1299
- https://doi.org/10.1126/science.6199844
Abstract
A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.This publication has 16 references indexed in Scilit:
- Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial CultivationScience, 1983
- Angiogenesis Inhibition and Tumor Regression Caused by Heparin or a Heparin Fragment in the Presence of CortisoneScience, 1983
- Protamine is an inhibitor of angiogenesisNature, 1982
- Migration of capillary endothelial cells is stimulated by tumour-derived factorsNature, 1980
- The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilageExperimental Cell Research, 1977
- The observation of heparin on endothelium after injectionThrombosis Research, 1976
- Enzymatic degradation of heparin-related mucopolysaccharides from the surface of endothelial cell culturesBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- TUMOR ANGIOGENESISThe Journal of cell biology, 1972
- Heparan sulfates of cultured cells. II. Acid-soluble and -precipitable species of different cell linesBiochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970