Pharmacological and Immunohistochemical Evidence for Serotonergic Modulation of Cholinergic Nucleus Basalis Neurons
- 1 May 1993
- journal article
- Published by Wiley in European Journal of Neuroscience
- Vol. 5 (5), 541-547
- https://doi.org/10.1111/j.1460-9568.1993.tb00519.x
Abstract
Identified electrophysiologically by low threshold bursts and transient outward rectification, cholinergic nucleus basalis neurons were recorded and labelled intracellularly in guinea‐pig basal forebrain slices. By means of a triple labelling immunofluorescent technique, serotonin‐immunoreactive fibres were visualized in close proximity to the soma and dendrites of the biocytin‐labelled, choline acetyl transferase (ChAT)‐immunoreactive cells. By bath application, 5‐hydroxytryptamine (5‐HT) produced a direct hyperpolarization of the identified cells which was mimicked by 5‐HT1A receptor agonists, suggesting that it may inhibit the tonic firing but also modulate the low threshold bursting of the cholinergic nucleus basalis neurons.Keywords
This publication has 46 references indexed in Scilit:
- A method for immunofluorescent demonstration of three coexisting neurotransmitters in rat brain and spinal cord, using the fluorophores fluorescein, lissamine rhodamine, and 7-amino-4-methylcoumarin-3-acetic acid.Journal of Histochemistry & Cytochemistry, 1990
- Brainstem afferents to the basal forebrain in the ratNeuroscience, 1988
- Three-color immunofluorescence histochemistry allowing triple labeling within a single section.Journal of Histochemistry & Cytochemistry, 1988
- Brainstem afferents to the magnocellular basal forebrain studied by axonal transport, immunohistochemistry, and electrophysiology in the ratJournal of Comparative Neurology, 1988
- Evidence that serotonin mediates non-cholinergic neocortical low voltage fast activity, non-cholinergic hippocampal rhythmical slow activity and contributes to intelligent behaviorBrain Research, 1986
- Sleep-related neuronal discharge in the basal forebrain of catsBrain Research, 1986
- Cholinergic activation of the electrocorticogram: Role of the substantia innominata and effects of atropine and quinuclidinyl benzilateBrain Research, 1984
- The distribution and origin of serotonin‐containing fibers in the septal area: A combined immunohistochemical and fluorescent retrograde tracing study in the ratJournal of Comparative Neurology, 1982
- Norepinephrine and serotonin: Opposite effects on the activity of lateral geniculate neurons evoked by optic pathway stimulationExperimental Neurology, 1980
- THE ASCENDING CHOLINERGIC RETICULAR SYSTEM: NEOCORTICAL, OLFACTORY AND SUBCORTICAL PROJECTIONSBrain, 1967