α‐Guanidinoglutaric Acid in Cobalt‐Induced Epileptogenic Cerebral Cortex of Cats

Abstract

Guanidino compounds in Co-induced epileptogenic cerebral cortex of cats were fluorometrically analyzed by a JASCO G-520 guanidino compounds analyzer; an unknown high peak was observed in the chromatogram that was identical to the peak of authentic .alpha.-guanidinoglutaric acid. The substance was extracted from the Co focus tissue, converted into dimethylpyrimidyl derivative-butylester and analyzed by a GC/MS [gas chromatography-mass spectrometry] technique. The mass spectrum of the substance was identical to the dimethylpyrimidyl derivative of .alpha.-guanidinoglutaric acid butylester (M+ [MW of parent compound] = 365).