Competition between tetracycline and tRNA at both P and A sites of the ribosome of Escherichia coli
- 23 March 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 213 (2), 443-447
- https://doi.org/10.1016/0014-5793(87)81539-9
Abstract
Fluorescence anisotropy studies performed on 6‐demethylchlortetracycline, binding to the ribosome of E. coli in competition with tRNA at the P site or at both P and A sites, have provided a quantitative assessment in situ of the interaction of this antibiotic with the A site and have demonstrated that there is also an interaction between tetracycline and the P site.Keywords
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