Human monocyte/macrophage activation and interleukin 1 generation by biomedical polymers

Abstract

In vitro cell culture techniques were used to evaluate the effect of several clinically significant biomedical polymers on monocyte activation and Interleukin 1 (IL1) production. Isolated human peripheral blood monocytes were cultured in the presence of a panel of five biomedical polymers routinely used in a variety of clinical applications: Polyethylene (PE), silica-free polydimethylsiloxane (PDMS), woven Dacron fabric, expanded polytetrafluoroethylene (ePTFE) and the segmented polyurethane, Biomer. Monocytes generated IL1 in the presence of all five materials. Maximal levels of IL1 were generated at 24 h in monocyte-polymer cultures supplemented with serum and additionally stimulated with lipopolysaccharide (LPS). No difference was observed due to serum source. Results from cultures supplemented with fetal bovine serum were not significantly different from those obtained with human serum supplemented cultures. The thymocyte proliferative activity generated by monocytes in the presence of these biomedical polymers was neutralized by a specific polyclonal anti-IL1 antiserum. Statistically significant differences in IL1 production were observed between polymers, allowing their classification according to reactivity into high (Dacron, PE), intermediate (ePTFE) and low (Biomer, PDMS) reactive groups.