Type I procollagen gene expression in normal and early healing of the medial collateral and anterior cruciate ligaments in rabbits: An in situ hybridization study
- 1 May 1991
- journal article
- research article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 9 (3), 374-382
- https://doi.org/10.1002/jor.1100090309
Abstract
The purpose of this study was to compare the levels of procollagen type I messenger RNA (mRNA) in normal and healing medial collateral ligament (MCL) and anterior cruciate ligament (ACL) in a rabbit model. Our method of injury involved a surgical model with identical partial lacerations in the midsubstance of the MCL and ACL. Paraffin sections of normal ligaments, and ligaments 3, 7, 14, and 28 days postlaceration were studied by in situ hybridization to compare and follow the level of type I procollagen mRNA in the two ligaments. A complementary DNA (cDNA) probe corresponding to α1(I) procollagen mRNA was labeled with [32P]d-CTP. After hybridization, autoradiography, and staining of the sections, the level of procollagen mRNA was assessed by microscopic examination. A higher level of procollagen mRNA was consistently detected in normal MCL than in normal ACL, suggesting higher collagen synthetic activity in the MCL. At the injury sites of the MCL and ACL, the levels of type I procollagen mRNA increased at all postlaceration periods, reaching its highest level at 14 days postsurgery. The MCL healing site had a considerably higher level of procollagen mRNA than the ACL healing site (i.e., injury site) at all postoperative intervals. The results demonstrate that procollagen mRNA levels in MCL tissue are higher than those in ACL tissue under normal conditions, as well as in response to injury. The differences in the procollagen mRNA levels of MCL and ACL may reflect the synthesis of collagen in these tissues, and may contribute to the differences in their healing capacities.Keywords
This publication has 21 references indexed in Scilit:
- The early effect of high molecular weight hyaluronan (hyaluronic acid) on anterior cruciate ligament healing: An experimental study in rabbitsJournal of Orthopaedic Research, 1990
- Regulation of collagen synthesis in fibroblasts within a three-dimensional collagen gelExperimental Cell Research, 1988
- Localization of types I, II, and III collagen mRNAs in developing human skeletal tissues by in situ hybridizationThe Journal of cell biology, 1987
- New experimental procedures to evaluate the biomechanical properties of healing canine medial collateral ligamentsJournal of Orthopaedic Research, 1987
- Collagen Alteration in Medial Collateral Ligament Healing in a Rabbit ModelConnective Tissue Research, 1987
- Medial collateral ligament healingThe American Journal of Sports Medicine, 1983
- Tendons and ligaments: A morphological and biochemical comparisonJournal of Orthopaedic Research, 1983
- Natural history of anterior cruciate tearsThe American Journal of Sports Medicine, 1979
- Isolated tear of the anterior cruciate ligament: 5-year follow-up studyThe American Journal of Sports Medicine, 1976