Immunoprecipitins in human schistosomiasis detected with adult worm antigens released by 3M KC1
- 1 March 1977
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Helminthology
- Vol. 51 (1), 71-72
- https://doi.org/10.1017/s0022149x00007276
Abstract
Adult Schistosoma mansoni extracts obtained by agitation in 3 M KCl (EKCl) and extracts obtained by homogenization in saline (EHOM) were compared. using sera from 97 schistosome patients from Brazil with hepatointestinal disease and viable eggs in the stools. The 2 extracts were studied by immunoelectroosmophoresis (IEOP) and immunodifusion (ID) methods. EKCl extracts showed more immunoprecipitation lines than EHOM extracts when tested by IEOP against the human sera; 3 M KCl possibly released antigens not present in the saline extracts. Another possibility was that 3 M KCl potentiated antigenic determinants during the extraction procedure. The percentage of positive sera showing immunoprecipitation detected by IEOP seemed dependent on the method of extraction, while with ID there was no difference; this could not be explained.This publication has 7 references indexed in Scilit:
- Immunoelectroosmophoresis as a Rapid Method for Detecting Soluble Antigenic Fraction of Schistosoma mansoniJournal of Parasitology, 1975
- Immunodiagnosis of human schistosomiasis using different immunoprecipitation techniquesZeitschrift Fur Parasitenkunde-Parasitology Research, 1975
- Schistosoma mansoni: Antigenic heterogeneity of excretions and secretionsExperimental Parasitology, 1974
- Protein composition and protein synthesis in the surface membranes ofSchistosoma mansoniParasitology, 1972
- Immunoprecipitins in Schistosoma mansoni infections: II. Cercopithecus sabaeus and Macaca mulatta monkeysExperimental Parasitology, 1967
- COMPARATIVE IMMUNOCHEMISTRY OF LARVAL AND ADULT FORMS OF SCHISTOSOMA MANSONI*Annals of the New York Academy of Sciences, 1963
- ANALYSIS OF HELMINTH ANTIGENS (ECHINOCOCCUS GRANULOSUS AND SCHISTOSOMA MANSONI) BY AGAR GEL METHODSAnnals of the New York Academy of Sciences, 1963