FSH AND LH STIMULATION OF ORNITHINE DECARBOXYLASE ACTIVITY: STUDIES WITH PORCINE GRANULOSA CELLS IN VITRO
- 1 October 1977
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 101 (4), 1335-1338
- https://doi.org/10.1210/endo-101-4-1335
Abstract
Under defined conditions in vitro FSH [follicle stimulating hormone] and LH [luteinizing hormone] caused a dose-dependent stimulation of ornithine decarboxylase (ODC) activity in granulosa cells isolated from porcine ovarian follicles. In cells from small follicles, FSH was at least twice as potent an inducer of the enzyme activity as LH. The cells from medium-sized follicles were more responsive to both hormones than cells from small follicles. The cells from medium-sized follicles were aporoximately equally responsive to maximal FSH and LH stimulation. Incubation of cells from either small follicles or medium-sized follicles with maximum effective doses of LH plus FSH caused no additive effects.This publication has 3 references indexed in Scilit:
- Ovarian ornithine decarboxylase induction: A specific and rapid in vivo bioassay of LHBiochemical Medicine, 1977
- The Porcine Ovarian Follicle: III. Development of Chorionic Gonadotropin Receptors Associated with Increase in Adenyl Cyclase Activity During Follicle MaturationEndocrinology, 1976
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951