The Coupling of Electron Flow to ATP Synthesis in Pea and Maize Mesophyll Chloroplasts
Open Access
- 1 September 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 68 (3), 610-615
- https://doi.org/10.1104/pp.68.3.610
Abstract
The rate of nonphosphorylating electron transport (in the absence of ADP and inorganic phosphate) in well-coupled (ATP/2e− = 0.9-1.1) maize mesophyll chloroplasts is not modulated by external pH (6.5-8.5), low levels of ADP or ATP, or energy transfer inhibitors, e.g. triphenyltin and Hg2+ ions. In contrast nonphosphorylating electron flow in pea chloroplasts is sensitive to alterations in medium pH, and to the presence of adenine nucleotides and energy transfer inhibitors in the assay medium. Although ATP is without effect on the rate of basal electron transport in maize chloroplasts, steady-state proton uptake is stimulated 3- to 5-fold by low levels of ATP. These results suggest that differences may exist in the manner in which the coupling factor complex controls proton efflux from the intrathylakoid space in C3 and C4 mesophyll chloroplasts.This publication has 21 references indexed in Scilit:
- A Proposed Mechanism for the Stimulatory Effect of Bicarbonate Ions on ATP Synthesis in Isolated ChloroplastsPlant Physiology, 1980
- Proton efflux through the chloroplast ATP synthase (CF 0 · CF 1 ) in the presence of sulfhydryl-modifying agentsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1980
- Hg2+-induced turnover of the chloroplast ATP synthetase complex in the absence of ADP and phosphateFEBS Letters, 1978
- Development of Photosystem I and Photosystem II Activities in Leaves of Light-grown Maize (Zea mays)Plant Physiology, 1977
- A simple, quantitative approach to the coupling of photophosphorylation to electron flow in terms of proton fluxesBiochemistry, 1976
- Structure and function of chloroplast ATPaseBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1976
- Energy‐dependent release of adenine nucleotides tightly bound to chloroplast coupling factor CF1FEBS Letters, 1976
- Conformational changes in coupling factor 1 may control the rate of electron flow in spinach chloroplastsBiochemical and Biophysical Research Communications, 1975
- Tightly bound nucleotides of the energy-transducing ATPase of chloroplasts and their role in photophosphorylationBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1975
- Studies on the energy coupling sites of photophosphorylation. I. Separation of Site I and Site II by partial reactions of the chloroplast electron transport chainBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1973