Adaptation of the Propionic-Acid Bacteria to Vitamin B 1 Synthesis Including a Method of Assay

Abstract
Propionibacterium pentosaceum when transferred continuously on a basal medium free of vitamin B1 can be trained to synthesize this vitamin to satisfy its growth requirements. A technique for the assay of vitamin B1 is described which is based on the increased anaerobic pyruvate metabolism of vitamin B1-deficient cells of P. pentosaceum occurring on the addition of the vitamin.

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