Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-14C and Potential Intermediates
- 1 July 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 42 (7), 941-945
- https://doi.org/10.1104/pp.42.7.941
Abstract
The isotope competition method with glucose-U-14C as a carbon source has been used to determine whether or not selected compounds contribute carbon to the biosynthesis of protein amino acids in cells derived from Paul''s Scarlet Rose. Of 48 compounds tested, 15 contributed carbon to protein amino acids. The results show for the first time that homoserine is an intermediate in threonine biosynthesis; that homoserine, cysta-thionine and homocysteine are intermediates in methionine biosynthesis and that histidinol is an intermediate in histidine biosynthesis in plant cells.Keywords
This publication has 21 references indexed in Scilit:
- Enzymatic synthesis of cystathionine by extracts of spinach, requiring O-acetylhomoserine or O-succinylhomoserineBiochemical and Biophysical Research Communications, 1966
- Biosynthesis of Protein Amino Acids in Plant Tissue Culture II Further Isotope Competition Experiments Using Protein Amino AcidsPlant Physiology, 1966
- Effect of 3-Amino-1,2,4-Triazole on Histidine Metabolism in AlgaePlant Physiology, 1966
- Aromatic metabolism in plants I. A study of the prephenate dehydrogenase from bean plantsBiochimica et Biophysica Acta (BBA) - General Subjects, 1966
- Diaminopimelate decarboxylase of Lemna perpusilla: Partial purification and some propertiesBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966
- The biosynthesis of protein amino acids in plant tissue culture I. Isotope competition experiments using glucose-U-C14 and the protein amino acids.Plant Physiology, 1965
- Amino Acid Metabolism in Young Pea SeedlingsPlant Physiology, 1965
- O-Succinylhomoserine as an Intermediate in the Synthesis of Cystathionine by Escherichia coliJournal of General Microbiology, 1964
- Purification of ornithine transcarbamylase from pea seedlingsArchives of Biochemistry and Biophysics, 1964
- Succinic Ester and Amide of Homoserine: Some Spontaneous and Enzymatic ReactionsScience, 1964