DNA SEQUENCING REPORT - DNA SEQUENCING SEPARATIONS IN CAPILLARY GELS ON A MODIFIED COMMERCIAL DNA SEQUENCING INSTRUMENT
- 1 July 1990
- journal article
- research article
- Vol. 9 (1), 74-+
Abstract
DNA sequencing separations of standard DNA fragments of known sequence have been achieved in small diameter capillary gels electrophoresed and analyzed in parallel in a modified commercial DNA sequencer instrument. DNA sequencing in terms of base-calling accuracy is comparable to conventional slab gels; however, the separations in the capillary were performed somewhat faster and required less sample than those in the slab gel. Advantages of this approach vs. separations on conventional slab gels are discussed.This publication has 10 references indexed in Scilit:
- Analytical and micropreparative ultrahigh resolution of oligonucleotides by polyacrylamide gel high-performance capillary electrophoresisAnalytical Chemistry, 1990
- Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.Proceedings of the National Academy of Sciences, 1988
- Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.Proceedings of the National Academy of Sciences, 1988
- USE OF A CHEMICALLY MODIFIED T7 DNA-POLYMERASE FOR MANUAL AND AUTOMATED SEQUENCING OF SUPERCOILED DNA1988
- Multiplex DNA SequencingScience, 1988
- A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating DideoxynucleotidesScience, 1987
- Fluorescence detection in automated DNA sequence analysisNature, 1986
- Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.Proceedings of the National Academy of Sciences, 1983
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977