Role of phosphate in the regulation of the Pasteur effect in Saccharomyces cerevisiae

Abstract

The occurrence of the Pasteur effect [activation of glycolysis by anaerobiosis] in S. cerevisiae in several conditions was examined. In these conditions measurements of a series of metabolites potentially involved in the regulation of the effect were performed. These included, among others, adenine nucleotides, citrate, fructose 2,6-bisphosphate and phosphate. Only phosphate changed in a consistent way, increasing in anaerobiosis when the Pasteur effect occurred. With the availabile data, only phosphate may be considered as a regulator of the Pasteur effect in this microorganism.