Immobilization of enzymes on a microchannel surface through cross-linking polymerization
- 19 September 2005
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- No. 40,p. 5062-5064
- https://doi.org/10.1039/b510605b
Abstract
A novel and facile method for the preparation of an enzyme-immobilized microreactor has been developed in which enzymes are immobilized as an enzyme-polymer membrane formed on the inner wall of the microchannel by a cross-linking polymerization method; the resulting microreactor shows excellent reaction performance and stability against denaturating agents.Keywords
This publication has 19 references indexed in Scilit:
- Why the move to microfluidics for protein analysis?Current Opinion in Biotechnology, 2004
- Preparation of functionalized nanostructures on microchannel surface and their use for enzyme microreactorsChemical Engineering Journal, 2004
- A simple method for surface modification of microchannelsNew Journal of Chemistry, 2003
- Modified micro-space using self-organized nanoparticles for reduction of methylene blueChemical Communications, 2003
- High Throughput Kinetic Investigations of Asymmetric Hydrogenations with MicrodevicesAdvanced Synthesis & Catalysis, 2003
- Conformational changes of enzymes adsorbed at liquid– solid interface: Relevance to enzymatic activityBiopolymers, 2002
- Development of Self-Heating Microreactor for Catalytic Reactions.JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 2001
- Surface field of forces and protein adsorption behavior of poly(hydroxyethylmethacrylate) films deposited from plasmaJournal of Biomedical Materials Research, 1995
- Cross-linked enzyme crystals as robust biocatalystsJournal of the American Chemical Society, 1992
- Étude du mécanisme d'établissement des liaisons glutaraldéhyde-protéinesBiochimie, 1976