The Differentiation of Live From Dead Sperms in Fowl Semen
- 1 January 1959
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 34 (1), 5-7
- https://doi.org/10.3109/10520295909114637
Abstract
A combined stain solution is made by dissolving 0.1 gm bromphenol blue and 0.2 gm nigrosin in 100 ml of a M/15 buffer solution of KH2PO4 and Na2HPO4 adjusted to pH 7.5. This staining solution was used to prepare stained fowl semen smears. Such smears give stable differentiation of live from dead sperms. The dead sperms are stained with a dark violet color while the live ones are not stained.Keywords
This publication has 1 reference indexed in Scilit:
- Fowl semen as collected by the massage methodThe Journal of Agricultural Science, 1957