An Improved Immunolabeling Method for Microtubular Cytoskeleton in Poplar (Populus nigraL) Free Nuclear Endosperm
- 1 January 1995
- journal article
- research article
- Published by Informa UK Limited in Biotechnic & Histochemistry
- Vol. 70 (5), 252-257
- https://doi.org/10.3109/10520299509108202
Abstract
We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde concentration in the fixative and the ethanol dehydration retains β-tubulin antigenicity and the former improves preservation of tissue structure. An ethanol-free embedding method is recommended for immunocytochemical studies of ethanol sensitive target proteins.Keywords
This publication has 19 references indexed in Scilit:
- Endosperm Development in Barley: Microtubule Involvement in the Morphogenetic Pathway.Plant Cell, 1994
- Microtubule Components of the Plant Cell CytoskeletonPlant Physiology, 1994
- Cellular Tensegrity: Exploring How Mechanical Changes in the Cytoskeleton Regulate Cell Growth, Migration, and Tissue Pattern during MorphogenesisInternational Review of Cytology, 1994
- Evaluation of fixative composition, fixative storage, and fixation duration on the fine structure and volume of root‐cell nucleoliBiology of the Cell, 1993
- Configuration of actin microfilaments during sporangium development inAchlya bisexualis: comparison of two staining protocolsProtoplasma, 1992
- Improvements in immunostaining samples embedded in methacrylate: localization of microtubules and other antigens throughout developing organs in plants of diverse taxaPlanta, 1992
- A convenient synthesis of hindered amines and α-trifluoromethylamines from ketonesTetrahedron Letters, 1990
- Visualization of microtubules in interphase and mitotic plant cells of Haemanthus endosperm with the immuno-gold staining method.Proceedings of the National Academy of Sciences, 1982
- Theoretical and practical aspects of glutaraldehyde fixationPublished by Springer Science and Business Media LLC ,1973
- A practical manual of medical and biological staining techniquesPublished by Biodiversity Heritage Library ,1956