Identification of mycoplasma colonies by immunobinding
- 1 April 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 23 (4), 783-785
- https://doi.org/10.1128/jcm.23.4.783-785.1986
Abstract
An immunobinding assay was developed to identify mycoplasma colonies on agar in pure and mixed cultures. Mycoplasma colonies on agar were transferred to nitrocellulose. The nitrocellulose was treated with specific rabbit antisera against mycoplasmas, peroxidase-conjugated goat anti-rabbit immunoglobulin G, 4-chloro-1-naphthol, and H2O2. Purple developed in the presence of specific reactions. The procedure was superior to epifluorescence.This publication has 14 references indexed in Scilit:
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