The Glycine Antagonist 7-Chlorokynurenic Acid Blocks the Effects of N-Methyl-d-Aspartate on Agonist-Stimulated Phosphoinositide Hydrolysis in Guinea-Pig Brain Slices
- 1 December 1990
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 55 (6), 1915-1919
- https://doi.org/10.1111/j.1471-4159.1990.tb05776.x
Abstract
Despite having no effect on basal phosphoinositide hydrolysis, N-methyl-D-aspartate (NMDA) inhibited carbachol-stimulated accumulation of 3H-inositol phosphates and enhanced that due to noradrenaline in guinea-pig cerebral cortex slices. The glycine antagonist 7-chlorokynurenic acid inhibited the effects of NMDA and this inhibition was reversed by glycine. The action of 7-chlorokynurenic acid was not mimicked by strychnine or HA 966 (1-hydroxy-3-aminopyrrolid-2-one). L-Glutamate also inhibited carbachol-stimulated accumulation of 3H-inositol phosphates, but this inhibition was not blocked by 7-chlorokynurenic acid. The data are consistent with glycine maintaining tonic control over NMDA receptor activity in guinea-pig brain.Keywords
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