Estimation of Genetic Variability of Vigna radiata Cultivars by RAPD Analysis
- 1 June 2004
- journal article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 48 (2), 205-209
- https://doi.org/10.1023/b:biop.0000033446.43495.0c
Abstract
DNA was isolated from 14 cultivars of Vigna radiata (L.) Wilczek and subjected to RAPD analysis using 14 random decamer primers. These cultivars revealed polymorphism with respect to RAPD markers and were subjected to hierarchical cluster analysis. A dendrogram was prepared based on these data. Analysis of banding patterns confirmed that two strongly aromatic cultivars IC1, IC4, were closely linked. But another aromatic cultivar, B1, formed a separate cluster. The high yielding cultivars were closely related to B1. The phylogenetic tree constructed by the neighbour joining method showed that RAPD results were correlated with morphological characters like plant height, leaf and seed size, seed colour, etc.Keywords
This publication has 12 references indexed in Scilit:
- Two genetic linkage maps of mungbean using RFLP and RAPD markersAustralian Journal of Agricultural Research, 2000
- Isozyme and RAPD Analysis of the Genetic Diversity Within and BetweenVigna luteolaandV. marinaAnnals of Botany, 1997
- Identification of Poinsettia Cultivars Using RAPD MarkersHortScience, 1997
- Estimation of heterozygosity in Ribes nigrum L. using RAPD markersGenetica, 1996
- Using Polymerase Chain Reaction-based DNA Amplification to Fingerprint North American Potato CultivarsHortScience, 1996
- Genetic variation and cultivar identification of Jamaican yam germplasm by random amplified polymorphic DNA analysisEuphytica, 1995
- A simple and rapid method for the preparation of plant genomic DNA for PCR analysisNucleic Acids Research, 1991
- Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain ReactionCold Spring Harbor Symposia on Quantitative Biology, 1986
- Mathematical model for studying genetic variation in terms of restriction endonucleases.Proceedings of the National Academy of Sciences, 1979
- Genetic Distance between PopulationsThe American Naturalist, 1972