Über eine mucoidähnliche Substanz aus Serum

Abstract

The serum was diluted with 2 vols. H2O, heated for 10 min. on a boiling H2O bath, and the mixture depro-teinized by adding 2 vols. of 25% sulfosalicylic acid soln. The filtrate was dialyzed against H2O, concentrated in vacuo at 35[degree], and treated with 2 vols. alc. After the mixture had stood 1 day in the ice box, the precipitate formed was washed with alc. and ether and dried. The product was the mucoid-like substance, fraction I. Fraction II was obtained by increasing the conc. of alc. in the above mother liquor to 90%. The yields of fractions I and II in mg. % for normal, cancer and gravid human serum, and normal and melanoma horse serum, were respectively: 10.1, 4.2; 29.8, 5.6; 8.55, 3.5; 8.26, 3.6; 12.8, 3.3. Both fractions gave positive Biuret, Millon, and Adamkiewicz protein reactions and positive carbohydrate reactions. Hydrolysis of the fractions with dilute HC1 gave glucosamine. The fractions evidently consisted of a mucoid-like substance with a relatively high S content. By polarographic analysis of the acid hydroly-sates, it was shown that the % cystine for normal, cancer, and inflammation serums for fraction I and II were respectively: 0.19, 0.34; 0.12, 0.08; 0.12, 0.17. The isoelectric point for the mucoid substance was at pH 3.4.