Snakin-1, a Peptide from Potato That Is Active Against Plant Pathogens
Open Access
- 1 January 1999
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 12 (1), 16-23
- https://doi.org/10.1094/mpmi.1999.12.1.16
Abstract
A new type of antimicrobial peptide, snakin-1 (SN1), has been isolated from potato tubers and found to be active, at concentrations <10 μM, against bacterial and fungal pathogens from potato and other plant species. The action of SN1 and potato defensin PTH1 was synergistic against the bacterium Clavibacter michiganensis subsp. sepedonicus and additive against the fungus Botrytis cinerea. Snakin-1 causes aggregation of both gram-positive and gram-negative bacteria. The peptide has 63 amino acid residues (M r 6,922), 12 of which are cysteines, and is unrelated to any previously isolated protein, although it is homologous to amino acid sequences deduced from cloned cDNAs that encode gibberellin-inducible mRNAs and has some sequence motifs in common with kistrin and other hemotoxic snake venoms. A degenerate oligonucleotide probe based on the internal sequence CCEECKC has been used to clone an SN1 cDNA. With the cDNA used as probe, one copy of the StSN1 gene per haploid genome has been estimated and expressi...Keywords
This publication has 35 references indexed in Scilit:
- Inactivation of the sapA to sapF Locus of Erwinia chrysanthemi Reveals Common Features in Plant and Animal Bacterial PathogenesisPlant Cell, 1998
- Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2The Plant Journal, 1997
- Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum.Plant Cell, 1997
- Antimicrobial Peptides from PlantsCritical Reviews in Plant Sciences, 1997
- Small cysteine-rich antifungal proteins from radish: their role in host defense.Plant Cell, 1995
- Peptide Antibiotics and Their Role in Innate ImmunityAnnual Review of Immunology, 1995
- Innate immunity of insectsCurrent Opinion in Immunology, 1995
- The defensive role of nonspecific lipid-transfer proteins in plantsTrends in Microbiology, 1995
- Developmental and pathogen‐induced expression of three barley genes encoding lipid transfer proteinsThe Plant Journal, 1993
- Defensins: Antimicrobial and Cytotoxic Peptides of Mammalian CellsAnnual Review of Immunology, 1993