Molecular weight estimation of immunoglobulin subunits of polyacrylamide gel.

  • 1 December 1971
    • journal article
    • Vol. 21 (6), 1065-71
Abstract
Molecular weights of immunoglobulin polypeptide chains can be accurately determined by dodecyl sulphate polyacrylamide gel electrophoresis. Non-alkylated immunoglobulin preparations can be used. Depending on the mobility of the polypeptide chain, a relatively narrow range of standard size should be used to calibrate the molecular weight plots; this is concluded from an evaluation of the molecular weight-mobility relationship of dodecyl sulphate-complexes of proteins in 10 per cent polyacrylamide gel.