Physical maps for Herpes simplex virus type 1 DNA for restriction endonucleases Hind III, Hpa-1, and X. bad

Abstract

It was proposed that the genome of herpes simplex virus type 1 (HSV-1) consists of 2 internal unique sequences, S and L, bounded by 2 sets of redundant sequences (P. Sheldrick and N. Berthelot, 1974). In this arrangement, terminal sequences (TRS and TRL) are repeated in an internal inverted form (IRS and IRL) and delimit S and L. A body of evidence has accumulated that suggests that S and L themselves are inverted, giving rise to 4 related forms of the HSV genome. In this study the ordering of restriction endonuclease fragments of HSV-1 DNA for physical maps was studied using molecular hybridizaiton techniques and the cleavage of isolated restriction endonuclease fragments with further restriction endonucleases. Physical maps for the fragments produced by Hind III, Hpa-1 and X. bad were constructed for the 4 related forms of the HSV-1 genome. TRS and IRS were between 3.5 .times. 106 and 4.5 .times. 106 daltons, TRL and IRL about 6 .times. 106 daltons, S about 8 .times. 106 to 9 .times. 106 daltons and L about 6.8 .times. 106 daltons.