Demonstration of specific heterochromatic segments in the orangutan (Pongo pygmaeus) by a distamycin/DAPI double staining technique
- 1 January 1979
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 24 (1), 7-14
- https://doi.org/10.1159/000131351
Abstract
Orangutan metaphase chromosomes, obtained from a female animal with trisomy 22, were stained by a recently developed distamycin/DAPI fluorescent technique which had been shown to differentiate specific C-bands on human chromosomes, as well as by other banding techniques. Distamycin/DAPI-brilliant fluorescence was observed in the short arm regions of acrocentric chromosomes 11–17, 22 and 23, and a brightly fluorescent paracentromeric band was seen on chromosome 8. A smaller amount of fluorescence was found adjacent to the centromere of most of the other chromosomes. Sequential distamycin/DAPI and Giemsa C-staining of the same metaphases revealed that the “hot-spots” on acrocentrics correspond to C-band material in their short arm. The less intensely fluorescent centric bands on other chromosomes coincide with centromeric C-bands. Reverse fluorescent staining of orangutan chromosomes with chromomycin A3 revealed brightly fluorescent regions at short arms of some acrocentrics probably confined to the satellite, either in a heteromorphic form (Nos. 11, 14, 17) or of similar size (No. 22).Keywords
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