Chloroplast DNA transcripts are encapsidated by tobacco mosaic virus coat protein
- 1 March 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (6), 1719-1723
- https://doi.org/10.1073/pnas.81.6.1719
Abstract
Preparations of tobacco mosaic virus contain pseudovirions, particles resembling virions but containing host rather than viral RNA. The encapsidated host [tobacco] RNA was found to be composed of discrete-sized species derived from a large portion of the chloroplast genome except that very little, if any, ribosomal RNA is present. Pseudovirions contain the same chloroplast DNA transcripts as those detected in extracts from uninfected leaves, although not always in the same relative amounts. Several strains of tobacco mosaic virus were tested and all were found to contain pseudovirions, with the U2 strain containing more than the others.Keywords
This publication has 22 references indexed in Scilit:
- Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proceedings of the National Academy of Sciences, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- A messenger RNA for capsid protein isolated from tobacco mosaic virus-infected tissueVirology, 1976
- Efficient transcription of RNA into DNA by avian sarcoma virus polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- In vitro and in vivo translation of the ribonucleic acids of a cowpea strain of tobacco mosaic virusVirology, 1976
- Occurrence of short particles in beans infected with the cowpea strain of TMVVirology, 1976
- Messenger RNA for the coat protein of tobacco mosaic virusNature, 1976
- Methylmercury as a reversible denaturing agent for agarose gel electrophoresisAnalytical Biochemistry, 1976
- Partial characterization of virus-like particles in chloroplasts of plants infected with the U5 strain of TMVVirology, 1975
- Purification and Characterization of Adenosine Triphosphate: Ribonucleic Acid Adenyltransferase from Escherichia coliEuropean Journal of Biochemistry, 1973