The heterocysts of blue-green algae. III. Differentiation and nitrogenase activity

Abstract

The course of heterocyst development in Anabaena cylindrica was studied in relation to the ability to fix nitrogen. When non-differentiated filaments, grown in the presence of ammonium-N, were transferred into a medium free from combined nitrogen and incubated under photosynthetic conditions, the cellular C:N ratio increased from 4.5:1 to 8:1 before the percentage heterocyst frequency and nitrogenase activity reached a steady value. The initial stages of differentiation were observed 24 h after transfer into nitrogen-free medium, but nitrogenase activity was only detected when the formation of the first heterocysts was completed. The transformation of a vegetative cell into a heterocyst is characterized by the dissolution of storage granules, the deposition of a multilayered envelope, the breakdown of photosynthetic thylakoids and the formation of new membraneous structures. The latter appear to develop by the coalescence of small newly formed vesicles arising in regions of pre-existing thylakoids. The course of heterocyst development was paralleled by that of nitrogenase activity both under aerobic and anaerobic conditions. Anaerobic incubation enhanced heterocyst production as well as nitrogenase activity. The results suggest that nitrogenase synthesis in Anabaena cylindrica is associated with heterocyst formation and that the primary factor which may regulate both processes is the cellular C:N balance of the alga.