Temperature-sensitive mutants of Balb/3T3 cells. III. Hybrids betweents2 and other mouse mutant cells affected in DNA synthesis and correction ofts2 defect by human X chromosome

Abstract

Complementation studies were performed withts 2, a mouse 3T3 cell mutant temperature sensitive (ts) for cell and viral DNA synthesis. Thets phenotype is corrected by non -ts mouse or human cells and a non-DNA⁻ ts mutant. This gene had been localized to a region on the human X chromosome near the HPRT locus based on isozyme and karyotype analysis of hybrids. Unusually rapid loss and fragmentation of human chromosomes occurs in hybrids withts 2. Hybrids betweents 2 and other DNA⁻ ts mutants of mouse cells did not show complementation of the growth phenotype.