HORMONAL EFFECTS ON GLUCOSE-6-PHOSPHATE DEHYDROGENASE OF MOUSE MAMMARY GLAND
Open Access
- 1 March 1963
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 11 (2), 218-223
- https://doi.org/10.1177/11.2.218
Abstract
Methods are presented to measure glucose-6-phosphate dehydrogenase in 1 to 5 gamma frozen dried samples of mouse mammary duct and mammary fat. The effects of incubation time, temperature, pH, substrate and pyridine nucleotide concentration on the enzyme activity are reported. During lactation activity of this enzyme increases about 9-fold in mammary duct. There is no significant difference in activity during estrus and diestrus. Certain C17 and C20 ketosteroids, including pregnenolone and dehydroepiandrosterone, inhibit the glucose- 6-phosphate dehydrogenase activity of mouse mammary gland homo-genates and freeze dried sections of duct and fat Mouse and rat breast glucose-6-phosphate dehydrogenase is not inhibited by 3, 20-preg-nanedione, even though this compound causes marked inhibition of that of the adrenaL.Keywords
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