Partial physical map of human chromosome 21
- 1 November 1988
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 14 (6), 623-637
- https://doi.org/10.1007/bf01535316
Abstract
The long arm of human chromosome 21 has been analyzed with unique sequence DNA probes, using an expanded panel of somatic cell hybrids containing defined regions of the chromosome, and both standard and pulsed field gel electrophoresis. Each member of the hybrid cell panel contains either a normal chromosome 21, or one of 11 different translocations or deletions within the long arm. Together, these now include 11 breakpoints, defining 11 long arm regions. Thirty-two unique sequence probes have been localized to these regions by standard gel electrophoresis. Analysis by pulsed field gels indicates that 27 of these identify a total of 18 Not1 restriction fragments, which together account for approximately 17 million base pairs, over half the long arm. Five physical linkage groups have been identified, as well as patterns in the distribution of unique sequences and GC-rich chromosomal regions. This information can be correlated with that obtained by other methods and contributes to the construction of a detailed physical map of this chromosome.Keywords
This publication has 56 references indexed in Scilit:
- Preferential location of X-ray induced chromosome breakage in the R-bands of human chromosomesHereditas, 2009
- Genetic linkage map of human chromosome 21Genomics, 1988
- Construction of a cDNA clone containing the entire coding region of the human liver-type phosphofructokinaseBiochemical and Biophysical Research Communications, 1987
- Two Distinct Compositional Classes of Vertebrate Gene-Bearing DNA Stretches, Their Structures and Possible Evolutionary OriginDNA, 1987
- Cloning of the $beta; subunit of the leukocyte adhesion proteins: Homology to an extracellular matrix receptor defines a novel supergene familyCell, 1987
- Construction of a mini-chromosome by deletion and its mitotic and meiotic behaviour in fission yeastMolecular Genetics and Genomics, 1986
- Cytogenetics of Bloom's syndromeCancer Genetics and Cytogenetics, 1986
- A fraction of the mouse genome that is derived from islands of nonmethylated, CpG-rich DNACell, 1985
- A (6;21) translocation, unbalanced, 46 chromosomesCytogenetic and Genome Research, 1974
- Quantum yield of acridines interacting with DNA of defined base sequence: A basis for the explanation of acridine bands in chromosomesExperimental Cell Research, 1972