Effects of adiponectin on breast cancer cell growth and signaling
Open Access
- 8 January 2008
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 98 (2), 370-379
- https://doi.org/10.1038/sj.bjc.6604166
Abstract
Obesity is a risk factor for postmenopausal breast cancer. Adiponectin/Acrp30 is lower in obese individuals and may be negatively regulating breast cancer growth. Here we determined that five breast cancer cell lines, MDA-MB-231, MDA-MB-361, MCF-7, T47D, and SK-BR-3, expressed one or both of the Acrp30 receptors. In addition, we found that the addition of Acrp30 to MCF-7, T47D, and SK-BR-3 cell lines inhibited growth. Oestrogen receptor (ER) positive MCF-7 and T47D cells were inhibited at lower Acrp30 concentrations than ER-negative SK-BR-3 cells. Growth inhibition may be related to apoptosis since PARP cleavage was increased by Acrp30 in the ER-positive cell lines. To investigate the role of ER in the response of breast cancer cells to Acrp30, we established the MDA-ERα7 cell line by insertion of ER-α into ER-α-negative MDA-MB-231 cells. This line readily formed tumours in athymic mice and was responsive to oestradiol in vivo. In vitro, MDA-ERα7 cells were growth inhibited by globular Acrp30 while the parental cells were not. This inhibition appeared to be due to blockage of JNK2 signalling. These results provide information on how obesity may influence breast cancer cell proliferation and establish a new model to examine interactions between ER and Acrp30.Keywords
This publication has 59 references indexed in Scilit:
- Diet-induced obesity and mammary tumor development in relation to estrogen receptor statusCancer Letters, 2007
- Adiponectin actions in the cardiovascular systemCardiovascular Research, 2007
- Adiponectin as an anti-inflammatory factorClinica Chimica Acta; International Journal of Clinical Chemistry, 2007
- Adiponectin mediates antiproliferative and apoptotic responses in human MCF7 breast cancer cellsBiochemical and Biophysical Research Communications, 2006
- Effect of Obesity on Presentation of Breast CancerAnnals of Surgical Oncology, 2006
- Cloning of adiponectin receptors that mediate antidiabetic metabolic effectsNature, 2003
- Globular Adiponectin Protected ob/ob Mice from Diabetes and ApoE-deficient Mice from AtherosclerosisJournal of Biological Chemistry, 2003
- Oligomerization State-dependent Activation of NF-κB Signaling Pathway by Adipocyte Complement-related Protein of 30 kDa (Acrp30)Journal of Biological Chemistry, 2002
- Blood Growth Hormone-Binding Protein Levels in Premenopausal and Postmenopausal Women: Roles of Body Weight and Estrogen LevelsJournal of Clinical Endocrinology & Metabolism, 2001
- Point mutation of estrogen receptor (ER) in the ligand-binding domain changes the pharmacology of antiestrogens in ER-negative breast cancer cells stably expressing complementary DNAs for ERMolecular Endocrinology, 1992