Gas Chromatography as a Sampling Procedure for Thin-Layer or Paper Chromatography

Abstract

A new chromatographic technique is described in which the sample components emerging (in milligram quantities) from a gas liquid chromatographic (GLC) column are developed from the start line of the plate used in thin-layer chromatography (TLC) or of a sheet of chromatographic paper (PC). The plate or the paper is driven linearly with time or according to a special program. Thus, the whole method results in the separation of sample components according to two different characteristics: GLC separates according to the relative volatility of compounds (in the direction of the time axis) and TLC or PC separates according to the types of the functional groups (vertical to the time axis). After extraction of the separated material from the plate or paper, subsequent chromatographic separation by GLC on a suitable stationary phase can be carried out again, if necessary.