ADDITIONAL TUBERCULOUS ANTIBODIES DEMONSTRATED WITH ERYTHROCYTES SENSITIZED BY SUCCESSIVE ADSORPTIONS OF OLD TUBERCULIN AND FURTHER STUDIES ON THE ADSORPTIVE CAPACITIES OF TANNED CELLS

Abstract

Twelve successive adsorptions with normal sheep cells did not completely remove the polysaccharide antigens from old tuberculin (OT). The rate at which the various antigens are removed and the demonstration of others only on cells used for later adsorptions emphasizes the multiplicity and independence of the corresponding antibodies. The successive adsorptions of OT with sheep cells permits the demonstration of antibodies in tuberculous sera which probably are directed against antigens not detected by usual sensitizing procedures. Tanned and normal sheep cells adsorb lytic antigens from OT equally well, but the adsorption of agglutinogens is different.