Human intestinal mucosal mast cells: evaluation of fixation and staining techniques.
Open Access
- 1 August 1981
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 34 (8), 851-858
- https://doi.org/10.1136/jcp.34.8.851
Abstract
The staining properties of tissue mast cells are influenced by the method of fixation. Differences in fixation and staining techniques may explain the contradictory results in the published reports on the number of human mucosal mast cells (MMC) in the gastrointestinal mucosa in health and disease. We have examined the influence of fixatives on the staining properties of human MMC in operative biopsy specimens of human jejunum. Specimens were divided into pieces, each of which was fixed in one of the following fixatives: Carnoy's, basic lead acetate (BLA), Baker's, Bouin's, isotonic formol-acetic-acid (IFAA), 10% neutral buffered formalin, formol sublimate, and formol saline. Thereafter, tissues were paraffin-embedded and 5 micron sections were cut and stained with either astra-blue/safranin pH 0.3, or toluidine blue pH 0.5. Counts of the number of MMC/mm2 were obtained for each fixation method. The results show a critical influence of the fixative on the number of mast cells identified after staining. For example with astra-blue/safranin the mean MMC/mm2 count was 40 in formol-saline-fixed specimens, and 268 in Carnoy's-fixed specimens. In biopsies fixed with formalin-based fixatives, mast cells were more readily stained with toluidine blue. It is recommended that Carnoy's or BLA be used as the fixative for any light microscopic study of human MMC.This publication has 17 references indexed in Scilit:
- Gastrointestinal mast cells in health, and in coeliac disease and other conditions.Archives of Disease in Childhood, 1980
- The in Vitro Effect of Neurotensin on Human Jejunal Mast CellsScandinavian Journal of Gastroenterology, 1980
- A quantitative study of the influence of fixation on immunoperoxidase staining of rectal mucosal plasma cells.Journal of Clinical Pathology, 1980
- G-cell populations in resected stomachs from gastric and duodenal ulcer patientsGastroenterology, 1980
- THYMUS-DEPENDENT AND THYMUS-INDEPENDENT SUB-POPULATIONS OF INTESTINAL INTRA-EPITHELIAL LYMPHOCYTES - GRANULAR SUB-POPULATION OF PROBABLE BONE-MARROW ORIGIN AND RELATIONSHIP TO MUCOSAL MAST-CELLS1980
- Duodenal endocrine cells in adult coeliac disease.Gut, 1979
- The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions.The Journal of Experimental Medicine, 1978
- Immunofluorescent localization of a serine protease in rat small intestine.Proceedings of the National Academy of Sciences, 1978
- Does heparin occur in mucosal mast cells of the rat small intestine?Journal of Histochemistry & Cytochemistry, 1977
- The copper phthalocyanin dye ?Astrablau? and its staining properties, especially the staining of mast cellsHistochemistry and Cell Biology, 1960