Isolation and characterization of catabolite repression-resistant mutants of Escherichia coli
- 1 October 1977
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 23 (10), 1384-1393
- https://doi.org/10.1139/m77-207
Abstract
A method has been developed for the isolation of Escherichia coli mutants which are resistant to catabolite repression. The method is based on the fact that a mixture of glucose and gluconate inhibits the development of chemotactic motility in the wild type, but not in the mutants. A motile E. coli strain was mutagenized and grown in glucose and gluconate. Mutants which were able to swim into a tube containing a chemotactic attractant (aspartic acid) were isolated. Most of these mutants were able to produce β-galactosidase in the presence of glucose and gluconate and were normal in their ability to degrade adenosine 3′,5′-cyclic monophosphate. Some of these mutants were defective in the glucose phosphotransferase system.This publication has 2 references indexed in Scilit:
- Evidence against the involvement of adenosine 3′,5′-cyclic monophosphate in glucose inhibition of β-galactosidase induction in Bacillus megateriumCanadian Journal of Biochemistry, 1976
- Inducible System for the Utilization of β-Glucosides in Escherichia coli I. Active Transport and Utilization of β-GlucosidesJournal of Bacteriology, 1967