Usefulness of the stomacher in a microbiological regulatory laboratory

Abstract

The relative efficiency of the Waring blender, the Stomacher 400 and the Stomacher 3500 for preparing food samples for microbiological analysis was studied. Comparative aerobic plate count (APC) values were determined on 671 samples, representing 30 categories of foods. Of the 26 categories of nonfatty foods, the blender gave significantly higher geometric mean APC values than those given by the Stomacher 400 and the Stomacher 3500 in 65 and 69% of the categories, respectively. In a comparison of the 2 stomacher models, the Stomacher 400 gave significantly higher geometric mean APC values than those given by the Stomacher 3500 in 73% of the food categories. Addition of Tween 80 to 4 categories of fatty foods at concentrations of 0.5, 1.0 and 2.0% did not raise the APC values given by either model of stomacher to the levels given by the Waring blender. Overall, the efficiency of both models of stomacher, relative to the blender and to each other, was specific and depended upon the particular food being analyzed.