A new anatomy of the prestalk zone in Dictyostelium
- 1 July 1989
- journal article
- Published by Springer Nature in Nature
- Vol. 340 (6229), 144-146
- https://doi.org/10.1038/340144a0
Abstract
The characteristic structure of the mature Dictyostelium culminant is created by the regionalized cellular differentiation and directed movement of prestalk cells. The front prestalk zone of the migratory slug has previously been considered to be a homogeneous tissue. Here we demonstrate, however, the existence of multiple classes of prestalk cells located in different parts or the slug anterior. The pDd56 and pDd63 genes encoding closely related extracellular matrix proteins are dependent for their expression upon DIF-1, the specific stalk-cell inducer. We have fused the promoters of the two genes to a modified chloramphenicol acetyltransferase (cat) gene to produce immunologically detectable proteins which localize to the cell nucleus. These two markers define three distinct kinds of 'prestalk' cells. One class, which we term 'prestalk A' cells, expressed the pDd63 gene. 'Prestalk B' cells express pDd56 and may also express the pDd63 gene. A third class, which we term 'prestalk 0' cells, expresses neither marker.Keywords
This publication has 23 references indexed in Scilit:
- Identification and localization of proteins encoded by two DIF-inducible genes of DictyosteliumDevelopmental Biology, 1988
- Structure and function of SV40 large-T antigenPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1987
- Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideumNature, 1987
- Direct induction of dictyostelium prestalk gene expression by DIF provides evidence that DIF is a morphogenCell, 1987
- Structural and functional characterization of aDictyostetiumgene encoding a DIF inducible, prestalk-enriched mRNA sequenceNucleic Acids Research, 1987
- A short amino acid sequence able to specify nuclear locationCell, 1984
- A possible morphogen controlling differentiation in DictyosteliumNature, 1983
- Cell patterning in DictyosteliumPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1981
- Cell differentiation without morphogenesis in Dictyostelium discoideumNature, 1976
- The Pattern of Differentiation in Amoeboid Slime MoldsThe American Naturalist, 1952